Anti-THEMIS antibodies are used in the immunodetection of the protein thymocyte selection associated. In humans, the canonical protein has a reported length of 641 amino acid residues and a mass of 73.5 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 4 different isoforms have been reported for this protein. It is notably expressed in the spleen, rectum, lymph node, duodenum, and colon. A member of the Themis protein family, THEMIS is known to play an important role in late thymocyte development by controlling both positive and negative T-cell selection.
The THEMIS marker can be used to identify Deep-Layer Intratelencephalic Neurons.* Synonyms for this target antigen include GRB2-associated protein, signaling phosphoprotein specific for T cells, thymocyte selection pathway associated, thymocyte-expressed molecule involved in selection, and protein THEMIS. THEMIS gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of THEMIS antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, Flow Cytometry is also a common application.
*HuBMAP Human Reference Atlas v1.4