Anti-ACOT4 antibodies are used in the immunodetection of the protein acyl-CoA thioesterase 4. In humans, the canonical protein has a reported length of 421 amino acid residues and a mass of 46.3 kDa. Its subcellular localization is in the peroxisomes. It is reported to be highly expressed in the liver and kidney and weaker expression in the placenta, heart, and muscle. A member of the C/M/P thioester hydrolase protein family, ACOT4 is known to catalyze the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Synonyms for this target antigen include PTE-2b, acyl-coenzyme A thioesterase 4, peroxisomal acyl coenzyme A thioester hydrolase Ib, peroxisomal acyl-CoA thioesterase 2B, and peroxisomal succinyl-coenzyme A thioesterase. ACOT4 gene orthologs have been reported in the mouse, rat and chimpanzee species. Western Blot is the most common application for the ACOT4 antibodies listed below. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.