Anti-E2F4 antibodies are used in the immunodetection of the protein E2F transcription factor 4. In humans, the canonical protein has a reported length of 413 amino acid residues and a mass of 44 kDa. Its subcellular localization is in the nucleus. It is reported to be found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. A member of the E2F/DP protein family, E2F4 is reported to be a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include E2F transcription factor 4, p107/p130-binding, p107/p130-binding protein, and transcription factor E2F4. E2F4 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.