Anti-SFN antibodies are used in the immunodetection of the protein stratifin. In humans, the canonical protein has a reported length of 248 amino acid residues and a mass of 27.8 kDa. Its subcellular localization is in the nucleus, cytoplasm, and is secreted. Up to 2 different isoforms have been reported for this protein. It is notably expressed in the skin, oral mucosa, esophagus, cervix, and caudate. A member of the 14-3-3 protein family, SFN is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Post-translational modifications have been described, including ubiquitination.
The SFN marker can be used to identify Type 3 Medullary Thymic Epithelial Cells.* Synonyms for this target antigen include 14-3-3 protein sigma, epithelial cell marker protein 1, and YWHAS. SFN gene orthologs have been reported in the mouse, rat, bovine, chimpanzee and chicken species. Over 160 citations in the literature describe the use of SFN antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4