Anti-SEMA5A antibodies are used in the immunodetection of the protein semaphorin 5A. In humans, the canonical protein has a reported length of 1074 amino acid residues and a mass of 120.6 kDa. Its subcellular localization is in the membrane. It is notably expressed in many tissues, such as the epididymis and lung. A member of the Semaphorin protein family, SEMA5A is known to be involved with cell adhesion and cell to cell signaling. Post-translational modifications have been described, including glycosylation.
The SEMA5A marker can be used to identify Deep-Layer Corticothalamic And 6B Neurons.* Synonyms for this target antigen include semF, sema F, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, semaphorin F, and SEMAF. SEMA5A gene orthologs have been reported in the mouse, rat, bovine, zebrafish, chimpanzee and chicken species. A number of SEMA5A antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.
*HuBMAP Human Reference Atlas v1.4