Anti-MDFI antibodies are used in the immunodetection of the protein MyoD family inhibitor. In humans, the canonical protein has a reported length of 246 amino acid residues and a mass of 25 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is notably expressed in many tissues, such as the cerebral cortex, urinary bladder, and bronchus. A member of the MDFI protein family, MDFI is reported to inhibit the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Synonyms for this target antigen include I-mfa, inhibitor of MyoD family a, myogenic repressor I-mf, and I-MF. MDFI gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some MDFI antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.