Anti-SEMA5B antibodies are used in the immunodetection of the protein semaphorin 5B. In humans, the canonical protein has a reported length of 1151 amino acid residues and a mass of 125.9 kDa. Its subcellular localization is in the membrane. Up to 4 different isoforms have been reported for this protein. It is notably expressed in the hippocampus, cerebral cortex, and cerebellum. A member of the Semaphorin protein family, SEMA5B is believed to act as positive axonal guidance cues. Post-translational modifications have been described, including glycosylation. Synonyms for this target antigen include SemG, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B, and SEMAG. SEMA5B gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some SEMA5B antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. Immunocytochemistry and Immunohistochemistry are also common applications.