Anti-ACSF3 antibodies are used in the immunodetection of the protein acyl-CoA synthetase family member 3. In humans, the canonical protein has a reported length of 576 amino acid residues and a mass of 64.1 kDa. Its subcellular localization is in the mitochondria. A member of the ATP-dependent AMP-binding enzyme protein family, ACSF3 is known to catalyze the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. The ACSF3 gene has been associated with the disease, Combined malonic and methylmalonic aciduria. Synonyms for this target antigen include acyl-CoA synthetase family member 3, mitochondrial, malonyl-CoA synthetase, and malonate--CoA ligase ACSF3, mitochondrial. ACSF3 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some ACSF3 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.