Anti-rubicon autophagy regulator antibodies are used for the immunodetection of the protein encoded by the RUBCN gene. In humans, the canonical protein has a reported length of 972 amino acid residues and a mass of 108.6 kDa. Its subcellular localization is in the lysosomes. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to inhibit PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. The gene encoding this protein is implicated in Spinocerebellar ataxia. Other names for this target antigen include RUBICON, SCAR15, run domain Beclin-1-interacting and cysteine-rich domain-containing protein, RUN and cysteine rich domain containing beclin 1 interacting protein, and KIAA0226. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish and chicken species.