Fig 1: COL stimulates RUBICON‐VPS34‐RAC1 formation and accelerates LC3‐associated efferocytosis ARepresentative images of LC3‐associated phagocytosis after pHrodo‐SE labeled‐ACs incubated with GFP‐LC3 BMDMs at the indicated time points. BMDMs were pre‐treated with 3‐MA (5 mM) or not for 30 min, and COL (1 μM) was added for another 2 h followed by addition of ACs. Green: GFP‐LC3; Red: pHrodo‐SE. Scale bar, 10 μm.BQuantification of LAP efficiency in GFP‐LC3‐BMDMs after different treatment (n = 5).C, DCell lysates of BMDMs treated with ACs were subjected to co‐IP by a VPS34 or RUBICON antibody and followed by WB analysis using the indicated antibodies, respectively. BMDM were pre‐treated with WRW4 (30 μM) or COL (1 μM).ERepresentative images of RAW 264.7 cells that transiently transfected with GFP‐C1‐PLCdelta‐PH after treating with COL (1 μM) or not. In WRW4 treated groups, WRW4 (30 μM) were treated for 30 min before adding COL. Red arrow indicates the pseudopodia with high dynamics.FQuantification of filopodia length alteration in different groups using Image J.GQuantification of relative speed of filopodia movement in different groups using Image J. Values are shown in box‐and‐whisker plots and the line in the box corresponds to the median. The boxes go from the upper to the bottom quartiles, and the whiskers go from the minimum to the maximum value (n = 14–19).HRepresentative images of GFP‐LC3 BMDMs treated with ACs. BMDMs were pre‐stained with LysoTracker Red for 30 min and pre‐treated with WRW4 (30 μM) for another 30 min in WRW4‐treated groups. ACs were added after COL (1 μM) were treated for another 2 h.ILine profiles of intensity of GFP and LysoTracker Red fluorescent value along the yellow line in (H). Data information: Data are shown as mean ± SD. *P < 0.05, **P < 0.01. Multiple t‐test are followed by Bonferroni correction. Experimental numbers indicate biological replicates. Source data are available online for this figure.