Anti-UPF1 RNA helicase and ATPase antibodies are used for the immunodetection of the protein encoded by the UPF1 gene. In humans, the canonical protein has a reported length of 1129 amino acid residues and a mass of 124.3 kDa. Its subcellular localization is in the nucleus and cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. It is ubiquitously expressed across many tissue types. A member of the DNA2/NAM7 helicase protein family, it is known to be a RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Post-translational modifications have been described, including phosphorylation. Other names for this target antigen include NORF1, RENT1, UTF, pNORF1, smg-2, regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, and HUPF1. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.