Anti-Legumain antibodies are used in the immunodetection of the protein encoded by the LGMN gene. In humans, the canonical protein has a reported length of 433 amino acid residues and a mass of 49.4 kDa. Its subcellular localization is in the lysosomes. Alternative splicing is reported to yield 3 different isoforms for this protein. It is ubiquitously expressed across many tissue types. A member of the Peptidase C13 protein family, it is known to have a strict specificity for hydrolysis of asparaginyl bonds. Post-translational modifications have been described, including glycosylation. Other names for this target antigen include LGMN1, PRSC1, asparaginyl endopeptidase, cysteine protease 1, protease, cysteine 1, and AEP.