Anti-NOP53 antibodies are used in the immunodetection of the protein NOP53 ribosome biogenesis factor. In humans, the canonical protein has a reported length of 478 amino acid residues and a mass of 54.4 kDa. Its subcellular localization is in the nucleus. It is notably expressed at high levels in the heart and pancreas, moderate levels in the placenta, liver, skeletal muscle, and kidney, and low levels in the brain and lung. A member of the NOP53 protein family, NOP53 is reported to be a nucleolar protein, which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include PICT-1, PICT1, ribosome biogenesis protein NOP53, glioma tumor suppressor candidate region gene 2 protein, p60, and GLTSCR2. NOP53 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.