Anti-WASHC5 antibodies are used in the immunodetection of the protein WASH complex subunit 5. In humans, the canonical protein has a reported length of 1159 amino acid residues and a mass of 134.3 kDa. Its subcellular localization is in the ER and cytoplasm. It is ubiquitously expressed across many tissue types. A member of the Strumpellin protein family, WASHC5 is known to act as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. The WASHC5 gene has been associated with the disease, Spastic paraplegia. Synonyms for this target antigen include RTSC, RTSC1, SPG8, WASH complex subunit strumpellin, strumpellin, and KIAA0196. WASHC5 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.