Anti-Cingulin antibodies are used in the immunodetection of the protein encoded by the CGN gene. In humans, the canonical protein has a reported length of 1203 amino acid residues and a mass of 137.1 kDa. Its subcellular localization is in the cell junctions. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Cingulin protein family, it is suspected to be plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.