Anti-NUP54 antibodies are used in the immunodetection of the protein nucleoporin 54. In humans, the canonical protein has a reported length of 507 amino acid residues and a mass of 55.4 kDa. Its subcellular localization is in the nucleus and membrane. Up to 3 different isoforms have been reported for this protein. It is notably expressed in many tissues, such as the bone marrow and fallopian tube. A member of the NUP54 protein family, Nup54 is a known component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane. Post-translational modifications have been described, including O-glycosylation. Synonyms for this target antigen include 54 kDa nucleoporin, nucleoporin 54kD, nucleoporin 54kDa, and nucleoporin p54. NUP54 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of Nup54 antibodies have been mentioned in research publications and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.