Anti-RUBCN antibodies are used in the immunodetection of the protein rubicon autophagy regulator. In humans, the canonical protein has a reported length of 972 amino acid residues and a mass of 108.6 kDa. Its subcellular localization is in the lysosomes. Up to 3 different isoforms have been reported for this protein. It is notably expressed in the spleen, fallopian tube, bronchus, and bone marrow. RUBCN is reported to inhibit PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Synonyms for this target antigen include RUBICON, SCAR15, run domain Beclin-1-interacting and cysteine-rich domain-containing protein, RUN and cysteine rich domain containing beclin 1 interacting protein, and KIAA0226. RUBCN gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chicken species. Over 80 citations in the literature describe the use of RUBCN antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.