Anti-ADHFE1 antibodies are used in the immunodetection of the protein alcohol dehydrogenase iron containing 1. In humans, the canonical protein has a reported length of 467 amino acid residues and a mass of 50.3 kDa. Its subcellular localization is in the mitochondria. Up to 4 different isoforms have been reported for this protein. It is notably expressed in adult liver. A member of the Iron-containing alcohol dehydrogenase protein family, Adhfe1 is known to catalyze the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). Synonyms for this target antigen include Fe-containing alcohol dehydrogenase 1, alcohol dehydrogenase 8, alcohol dehydrogenase iron-containing protein 1, and hydroxyacid-oxoacid transhydrogenase, mitochondrial. ADHFE1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Some Adhfe1 antibodies may have been used in research and have associated citations. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.