Anti-Myocardin antibodies are used for the immunodetection of the protein encoded by the MYOCD gene. In humans, the canonical protein has a reported length of 938 amino acid residues and a mass of 102 kDa. Its subcellular localization is in the nucleus. Alternative splicing is reported to yield 3 different isoforms for this protein. It is found to be expressed in the heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. It is noted to be involved in the apoptotic pathway and cardiac muscle cell differentiation. Post-translational modifications have been described, including phosphorylation.
The myocardin marker can be used to characterize Vascular Smooth Muscle Cells and Deep-Layer Near-Projecting Neurons.* Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. A number of Myocardin antibodies have been mentioned in research publications and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.
*HuBMAP Human Reference Atlas v1.4