Anti-EPHB4 antibodies are used in the immunodetection of the protein EPH receptor B4. In humans, the canonical protein has a reported length of 987 amino acid residues and a mass of 108.3 kDa. Its subcellular localization is in the cell membrane. Up to 4 different isoforms have been reported for this protein. It is found to be highly expressed in the placenta but also detected in the kidney, liver, lung, pancreas, skeletal muscle and heart. A member of the Tyr protein kinase protein family, EphB4 is a known receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Post-translational modifications have been described, including phosphorylation and glycosylation.
The EPHB4 marker can be used to identify Respiratory Vein Endothelial Cells.* Synonyms for this target antigen include HFASD, HTK, LMPHM7, MYK1, TYRO11, ephrin type-B receptor 4, ephrin receptor EphB4, and CMAVM2. EPHB4 gene orthologs have been reported in the mouse, rat, bovine, frog and chimpanzee species.
*HuBMAP Human Reference Atlas v1.4