
The non-viral transfection of genetic material into cells, also known as gene delivery, is simplified by commercially available reagents and kits. The appropriate method for gene delivery depends on the type of cell and genetic material. Plasmid DNA, siRNA, proteins, and antibodies are transfected into cells using methods such as liposomes, calcium phosphate, and electroporation. Proficient stable or transient cell lines are generated using established cell lines, primary cells or stem cells with the appropriate transfection method. In vivo transfection reagents are offered with markers, such as Neomycin resistance. In addition to basic transfection kits, enhancement compounds can be used to increase the efficiency of the selected transfection treatment. A diverse range of transfection products is available to match the required gene delivery method.
New tech making inroads on traditional block-based instruments
read more
Tech is helping usher in a new era of personalized cancer treatment
read more
Our InvivO2 workstation is packed with innovative features that allow you to study even the most complex cell interactions under perfect physiological oxygen conditions. Whether you’re hoping to replicate the environment of blood vessels or lung ...
read more
The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
read more
I often transiently overexpress proteins in U2OS cells and found Lipofectamine 3000 to be the best transfection reagent for this cell line.
read more
Lipofectamine 3000 Transfection Reagent gives good transfection performance with improved application outcomes and reproducible results.
read more