In Vivo Transfection

Transfection Reagents Transfection reagents are used to introduce DNA or RNA into eukaryotic cells. Transfection reagents help overcome the barrier caused by the negative charges of both the nucleic acids and the cellular membrane by giving an overall positive charge to the DNA or RNA through the formation of transfection complexes. This interaction is necessary to trigger cellular uptake of the complexes and subsequent release from vesicles into the cytoplasm. Transfection reagents are often cationic lipids or polymers and include calcium phosphate and diethylaminoethyl (DEAE)-dextran. Visit the supplier pages for more product information.

As with most cases regarding transition from in vitro to in vivo, In Vivo transfection is technically complicated. Some factors to take into account include DNA/RNA stability, efficiency of delivery into the body, and tissue/organ specificity. Fortunately, commercial manufacturers have designed kits and reagents especially for DNA or RNA delivery into the live animal. Lipid and non-lipid formulations are available, and many feature low toxicity and high delivery efficiency. Supporting data and publication references are also provided.

In Vivo Transfection

Your search terms and filters produced no results.

Please use the Transfection Reagents search tool above to find what you're looking for.

Suggested Products

  • InvivO2 Physiological Cell Culture Workstations

    Our InvivO2 workstation is packed with innovative features that allow you to study even the most complex cell interactions under perfect physiological oxygen conditions. Whether you’re hoping to replicate the environment of blood vessels or lung tissue, the InvivO2 is the best tool for the job. Easy to use and adaptable to meet your needs, especially for cell culture applications that require carefully controlled oxygen regulation, InvivO2 offers accurate and stable user-defined environmental ...

  • ExPERT ATx™ Electroporation System

    Hold your research and proof of concept studies to the highest standards with reliable and efficient electroporation technology. Features: Achieve high efficiency and cell viability Rapidly transfect from 75 thousand to 700 million cells Seamlessly transition to clinical GMP platform Enjoy compatibility with all MaxCyte static electroporation Processing Assemblies Accelerate the development of your research with reliable, scalable and efficient performance
  • <<
  • >>

Recent Product Review

  • FemtoJet 4x

    The goal of our research is to create a more efficient metastatic prostate cancer GEMM and we will be using both the femtojet and transferman to inject virus cells into our current mouse model and have the cancer cells become more precise. In the past, you could not differentiate the cancer cells, and with this new method of injection, we are able to create single colonies of cancer cells. This technique of using microinjection is also helpful for work with gene targeting and IVF. The goal of this product is to ensure that virus reaches the tissue and produces metastatic disease. By using the product, instead of by hand, creates more reproducible spread of cancer. This product was chosen because they could help us obtain the results we wanted and were most cost effective.

  • 4D Nucleofection of HUVECs

    After struggling with transfection of HUVECs using standard methods, the Lonza 4D nucleofection system was a godsend. Using the system I am able to to get high transaction efficiency with high viability. This is far superior to liposome based transfections