Cloning and Expression Vectors

Molecular cloning and genetics studies require the use of vectors as a host or carrier system to transfer selected genetic components, namely DNA, to cells or organisms. Specialized host vectors are available for cloning and expression of a variety of genes from numerous species and of variable size fragments. Vector technology facilitates library construction, single gene analysis, protein expression, and basic molecular cloning. The elected vector system is influenced by downstream applications, such as PCR, gene transfection or gene transduction. Plasmids and viral vectors are two of the most common vector types used. The development of fluorescence protein marked vectors, like GFP (Green Fluorescent Protein), allows the detection of gene expression within live cells. Antibiotics resistance, localized expression, engineered selection markers and cloning site size should be taken into consideration when choosing an appropriate vector.