Glycine buffers are buffering solutions widely used in laboratory applications such as Western blot, chromatography, electrophoresis, enzyme assays, and cell culture. A zwitterionic compound, glycine has pKa values of 2.3-2.4 and 9.6-9.8. Glycine is used to prepare several types of buffers. Glycine-HCl, combined with hydrochloric acid, has a buffering pH range of 2.2 to 3.6. Tris-Glycine, which includes tris base, is often used in SDS-PAGE. Glycine reagent appears as a white solid powder that will form a clear solution when dissolved. It may slowly turn yellow over time, although storing the solution in the dark may slow the process. Visit the supplier page for more reagent information, such as details on purity and format.