One-Step Gel Staining with GelCode Blue

Stanford University
Department of Pathology
Postdoctoral Researcher

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Company:

Thermo Fisher Scientific

Product Name:

GelCode™ Blue Stain Reagent

Catalog Number:

24592

I study gene signaling pathways and routinely use SDS-PAGE to assess protein expression and purification. GelCode Blue provides a quick, ready-to-use way to visualize proteins without a lengthy destaining process.

Experimental Design and Results Summary

Application

Protein staining of polyacrylamide gels.

Starting Material

Protein samples separated by SDS-PAGE.

Tips

The dye settles in the bottom of the bottle. Therefore, mix the reagent bottle before dispensing.

Results Summary

I get clear protein bands with a straightforward staining workflow, making it useful for quickly checking protein expression and quality.

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Summary

The Good

One-step staining followed by water wash. No traditional methanol/acetic acid destaining is required

The Bad

None

The Bottom Line

A convenient and reliable Coomassie stain that saves time compared with traditional gel-staining workflows.

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