Resolves the CD24/CD38 Transitional B Cell Axis Cleanly

August 13, 2026

BlueskyReddit
Bioengineering
Rice University
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PerCP anti-human CD24 Antibody

Catalog Number:

311114

Clone Number:

Clone ML5

CD24 paired with CD38 defines the transitional B cell compartment in human samples, which is a subset we track alongside naive, memory, and plasmablast populations. Together with CD19, CD20, and CD27 it completes our B cell subset panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs and PBMC-derived B cells, freshly isolated, approximately one million cells per test in 100 µL of cold FACS buffer.

Primary Incubation

5 µL per test as supplied (100 tests, 400 µg/mL), in FACS buffer, 20–30 minutes at 4 °C protected from light, two washes.

Blocking Agent

uman TruStain FcX, 5 µL per test, 10 minutes at 4 °C prior to staining.

Secondary Incubation

N/A — directly conjugated primary.

Tertiary Incubation

N/A

Detection

Flow cytometry, PerCP excited off the 488 nm blue laser and collected in the ~677 nm channel. Analysis in FlowJo: singlets, live cells, CD19+, then CD24 versus CD38 for the transitional gate.

Results Summary

CD24 expression on B cells is graded rather than binary, which is exactly the point — the transitional compartment is defined by being CD24-high and CD38-high, so what you need is enough resolution to place cells confidently along a continuum. Clone ML5 delivered that, with the CD24-high population clearly resolved from intermediate and low. PerCP is a mid-brightness fluorophore, which suits a well-expressed marker like CD24, and it sits in a blue-laser channel that is often free once FITC and PE are assigned. The one real limitation is photostability: PerCP photobleaches faster than most alternatives under sustained laser exposure, so acquisition settings and sample handling matter more than usual.

Additional Notes

PerCP is photosensitive — keep samples in the dark and avoid long acquisition times or high laser power, since bleaching during a run produces an apparent signal drift that is easy to misread as biology. If you are running a very long acquisition, consider PerCP/Cy5.5 instead, which is more robust. The CD24/CD38 transitional gate is only as good as your CD38 staining, so titrate both together rather than separately. Store at 2–8 °C protected from light, do not freeze.

Image Gallery

Summary

The Good

Resolves the graded CD24 expression needed for transitional B cell gating; sits in an often-free blue-laser channel; standard clone.

The Bad

PerCP photobleaches under sustained illumination, so acquisition discipline matters.

The Bottom Line

Does what the transitional gate needs. Keep it in the dark, keep acquisitions short, and it performs.

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