Efficient Calcium Assay Kit

Uka Tarsadia University

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Quality of Results

Ease-of-Optimization

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Company:

Cayman

Product Name:

Calcium Assay Kit

Catalog Number:

701220

The aim of the experiment was to assess intracellular (Treg cells) and plasma calcium levels by calcium assay.

Experimental Design and Results Summary

Application

Colorimetric assay used to measure total calcium concentration in biological samples

Starting Material

Plasma, tissue homogenates and cell lysates

Tips

The kit must be stored at 4°C.

Results Summary

Calcium level in plasma and cytoplasmic extract of Treg cells was measured by calcium assay kit (Cayman, MI). In our experiments we precisely assessed intracellular and plasma calcium levels.

DOI or PMID #

DOI: 10.1111/imm.13538

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Summary

The Good

The kit can be useful for assessing calcium concentration in biological samples including plasma, as well as tissue homogenates and cell lysates. It detects total calcium in biological samples as low as 0.25 mg/dl. The kit is useful for the sufficient sample size (i.e. 192 wells)

The Bad

The procedure requires bit more time due to incubation and sample preparation but depends on the sample types used in the experiments.

The Bottom Line

Calcium Assay Kit (Cayman, MI) is very apt to assess calcium in biological samples as well as cytoplasmic extracts. Moreover, the protocol is user-friendly but optimization is needed due to different cell types of the experiments.

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