Invitrogen
F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ 488
SA5-10382-AF488
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We used this secondary in ICC to detect a primary Ab towards our viral protein target. The Ab showed robust signal and minimal off target signal in negative control samples.
ICC
Vero cells infected with Dengue-3
1:1000
Normal Donkey Serum
1:2000
None
Plate Reader
This secondary antibody was effective to use and detected the primary. We saw minimal background in the negative control wells.
N/A
Easy to use and fluoresces at correct wavelength
Can be used at 1:2000 or lower for good detection of abundantly expressed proteins.