Clear Postsynaptic Puncta Labeling for GABAergic Synapse Analysis

July 08, 2026

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SUNY UB
Research Scientist

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Company:

Synaptic Systems

Product Name:

Gephyrin Antibody

Catalog Number:

147021

Clone Number:

Info:Supplier Page

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We used this antibody to detect inhibitory postsynaptic structures in mouse prefrontal cortex. The antibody produced well-defined Gephyrin-positive puncta with good contrast and consistent staining across tissue samples. Combined with VGAT labeling, it was suitable for quantitative analysis of GABAergic synaptic marker pairs in relation to individual astrocyte territories.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

40-μm free-floating mouse prefrontal cortex brain sections

Primary Incubation

1:1000 overnight at 4°C

Blocking Agent

3% normal goat serum + 0.3% Triton X-100 in PBS, 1 hour at room temperature

Secondary Incubation

Corresponding Alexa Fluor–conjugated secondary antibody, 1:1000, 2 hours at room temperature

Tertiary Incubation

NA

Detection

Confocal microscopy (Leica SP8) and 3D image analysis using Imaris

Results Summary

The antibody produced discrete Gephyrin-positive puncta that could be reliably detected and quantified in confocal z-stacks. In combination with VGAT staining, it enabled analysis of inhibitory synaptic marker pairs, including puncta density and spatial relationships with reconstructed astrocyte surfaces.

DOI or PMID #

DOI: 10.1126/sciadv.adt2527

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Summary

The Good

Well-defined postsynaptic puncta, good contrast, consistent staining, and strong performance in multiplex experiments.

The Bad

Quantitative puncta analysis benefits from high-resolution z-stacks and standardized detection thresholds.

The Bottom Line

A dependable antibody for high-resolution imaging and quantitative analysis of inhibitory postsynaptic structures in mouse brain tissue.

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