Ideal PCR Master Mix for Complex PCR

Stanford University
Biochemistry
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

PLATINUM SUPERFI II GREEN Master mix

Catalog Number:

12369010

I have used this PCR master mix for GC-rich and long PCR. This is an exceptional master mix with high fidelity. This has added DNA gel loading dye, so I use it for gel purification of PCR products straight after PCR.

Experimental Design and Results Summary

Application

PCR

Starting Material

Isolated DNA sample containing the target sequence

Tips

None

Results Summary

I use this master-mix for complex PCRs. I use 60 degrees as universal annealing temperature. Also, i have amplified various vector backbones (often close to 10 kb) using this mastermix, and I have not noticed any errors.

DOI or PMID #

N/A

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Summary

The Good

Exceptional polymerase for complex PCRs.

The Bad

None.

The Bottom Line

I would highly recommend this master mix for GC-Rich fragments and long PCR products.

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