Cell Signaling Technology
NF-κB p65 Mouse mAb
#6956
L8F6
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I used this antibody to check the expression of Nf-kB p65 in human colorectal cancer cell lines Colo205 and HT29. Primary incubation at 1:1000 at 4 degrees O/N worked really well for this antibody and gave a good signal at around 65 KDa.
Western blot
Human colorectal cancer cell line Colo205 and HT29
1:1000, 4 degrees, O/N
5% non-fat milk
1:20000, 1h at RT
None
Amersham ECL and picture taken using Chemidoc
The antibody detects a very clear band at 65 KDa and showed both Colo205 and HT29 cells to be positive expressing cells with HT29 showing a higher expression.
N/A
Clear band, and no background.
Cost is higher but comparatively, still reasonable.
Best p65 antibody used.