Cell Signaling Technology
TFAM (D5C8) Rabbit mAb
8076S
D5C8
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The antibody sensitivity for Western blot analysis is exceptionally high in human cells, including mesenchymal stem cells, HUVECs, and some cancer cell lines. To detect the presence of TFAM protein, the antibody was diluted to 1:1000 in a 5% BSA-containing TBST solution and incubated overnight. The following day, the membrane was incubated with an HRP-conjugated rabbit antibody for 1 hour. The development of HRP resulted in the detection of the TFAM band just 20 seconds later, indicating the effectiveness and speed of the antibody in identifying the target protein.
Western Blot
Human cell (MSC, HUVEC)
1/1000 in 5% BSA/TBST for overnight
5% BSA
1/10000 HRP-Rabbit IgG for 1 hr in room temperature
N/A
HRP for 20 second exposure
To investigate TFAM expression, I used human cells such as MSC, SMC, HUVEC. After inducing TFAM expression, we purified total protein using a protease/phosphatase inhibitor cocktail contained in RIPA buffer. We loaded 20 μg of the purified protein onto an SDS-PAGE gel, and were able to detect the protein band of interest without any background interference. This successful detection of TFAM protein suggests the efficacy of our experimental approach.
Highly efficient and no background.
Too expensive.
Highly recommend this antibody to the researchers who has an interest to mitochondrial DNA replication study.