Kerafast
Anti-Puromycin
EQ0001
3RH11
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Browse Similar Products: Puromycin Antibodies
A mouse model with I4895T mutation in type 1 ryanodine receptor displays myopathy including muscle weakness and atrophy. In vivo protein synthesis with puromycin labeling showed decrease of newly synthesized proteins compared to WT.
Western Blot
Skeletal muscle
1:1000, 2hs at R.T (or overnight at cold room)
Bio-Rad EveryBlot Blocking buffer
1:10,000, 1 hour at room temperature (StarBright 700)
No tertiary incubation
Bio-Rad ChemiDoc MP Imaging System
This is the assay for the detection of newly synthesized proteins (SUnSET). IP injection of puromycin into mice for 35 min labels newly synthesized proteins with puromycin. Puromycin Ab detects these proteins.
10.1038/ncomms14659
N/A
Good for Western blot.
Nothing bad.
We tested only Western Blot.