Beckman Coulter
CD22-APC-A700, SJ10.1H11, 0.5 mL, ASR
A89311
SJ10.1H11
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Browse Similar Products: CD22 Antibodies
Typically, B cells are characterized by their positive expression of CD19 or CD20. However, with modern antibody therapy, e.g. Rituximab, CD20 can be lost on the surface of B cells. The same can happen with anti-CD19. Therefore, other B cell markers are needed. We decided to test this anti-CD22 antibody for this purpose.
Flow Cytometry
Human white blood cells
10 minutes at room temperature
1% FBS
None
FL-7 (Navios EX)
The results were encouraging. B cells stained positively even when the patient was treated with Rituxan. The signal was not very bright, but positive enough. Compensation was tough (due to bleeding into APC and APC-A750 channels), but doable.
N/A
Positive signal easily detected.
Spillover into nearby channels. Expensive.
This is a workable antibody, that will solve a lot of issues in identifying B cells in patients on mAb therapies.