Great Antibody for Western Blot

May 13, 2022

Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Ubiquitinylated protein, clone FK2

Catalog Number:

04-263

Clone Number:

FK2

Works for Western blot using human iPSC-derived Ecs and HEK 293 at 1:1000. I got faint bands but likely due to my sample and not the antibody itself.

Experimental Design and Results Summary

Applications

Western Blot

Sample

iPSCs

Primary Incubation

1:1000 overnight BSA

Blocking Agent

BSA

Secondary Incubation

1 hr, milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

I used this to determine the ubiquitination of my protein of interest after treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

No cons.

The Bottom Line

Recommend

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