Really Good FKBP12 Antibody for Western Blot

Integrative Physiology
Baylor College of Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-FKBP12 antibody

Catalog Number:

ab2918

Clone Number:

Polyclonal

I used this Ab for detecting FKBP12 in the skeletal muscle of FKBP12KO and WT control mice. This Ab is really good for western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lysates of skeletal muscle

Primary Incubation

2 hours at room temperature

Blocking Agent

EveryBlot blocking buffer

Secondary Incubation

1:10,000, 1 hour at room temperature (StarBright 700)

Tertiary Incubation

No tertiary treatment.

Detection

Bio-Rad ChemiDoc MP Imaging System

Results Summary

This Ab is highly specific for FKBP12 without any sign of non-specific interaction.

DOI or PMID #

10.1074/jbc.M114.586289

Additional Notes

N/A

Image Gallery

Summary

The Good

Highly specific.

The Bad

No bad thing.

The Bottom Line

This Ab was tested only by western blot.

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