abcam
Recombinant Anti-alpha smooth muscle Actin antibody
ab124964
EPR5368
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I did western blot and immunofluorescence staining with this antibody. It works with human cells and rat primary cells. The background of this research is to check the a-SMA expression on MRC-5 human lung fibroblast cell expression.
Immunofluorescence
MRC-5 human lung fibroblast cells
1:500 overnight
2% BSA 10% goat serum
30 mins - 2h
1:500 room temperature 2 h
Confocal microscope
The signal looks great.
N/A
React with Human Rat Mouse sample
Expensive
Antibody has great signal. It works for different assays. Would buy again.