SinoBiological
SPEG Antibody
12472-T16
N/A
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Speg is striated muscle enriched protein kinase. I used skeletal muscle from Speg KO and WT to specifically target the Speg protein. This Ab detected both beta (full-length form) and alpha (N-term deleted form).
Western Blot
Skeletal muscle from WT and Speg-KO mice
1:1000, 2hs at R.T (or overnight at cold room)
Bio-Rad EveryBlot Blocking buffer
1:50,000, 1hr at R.T using Bio-Rad goat-anti rabbit IgG StarBright blue 700
No tertiary incubation
Bio-Rad ChemiDoc MP Imaging System
Although some minor non-specific bands under the target band, this Ab showed the specific target Speg bands (both beta and alpha).
Good for Western blot.
Some minor non-specific bands.
This Ab is good for Western blot. However, it is not guaranteed for histology in our conditions.