Very Strong Band with OPA1 Antibody

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

OPA1 Antibody

Catalog Number:

D6U6N

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: OPA1 Antibodies

Used at a concentration of 1:1000, it provides very strong bands. I used this antibody in SHSY5Y with the purpose to evaluate mitochodria fusion. The antibody detects the two isoforms of OPA1 and is very strong when used 1:1000 in BSA 5% on a nitrocellulose membrane. I used a secondary antibody 1:5000. Predicted molecular weight is correct. Strongly recommended.

Experimental Design and Results Summary

Applications

Western Blot

Sample

SH-SY5Y

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

Very strong and clean bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very strong and clean bands.

The Bad

Has to be used as last antibody because the stripping solution do not remove its presence.

The Bottom Line

Overall very good product. Reliable.

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