Great Antibody to Detect ERK through WB

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb

Catalog Number:

4695S

Clone Number:

N/A

Info:Supplier Page View Product Specs

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We used this antibody to test for the expression of Erk in dendritic cells using western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Dendritic cells

Primary Incubation

1:1000

Blocking Agent

BSA

Secondary Incubation

Anti rabbit-HRP ; 1:1000

Tertiary Incubation

None

Detection

ECL

Results Summary

Strong specific signals after few seconds of exposition. Any background. High quality results.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Cheap in cost, specific and easy to detect.

The Bad

Nothing

The Bottom Line

Perfect antibody to work with.

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