Measuring PECAM1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Novus Biologics

Product Name:

CD31/PECAM-1 Antibody (MEC13.3)

Catalog Number:

NB600-562

Clone Number:

N/A

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes. PECAM-1 present within the endothelial intercellular junctions, and supporting endothelial cell migration. Here we checked the time course effect of PECAM1 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent decrease of PECAM1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific and strong signal

The Bad

None

The Bottom Line

This antibody woks great for western blot. Highly recommended.

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