Time Course Effect of Chemoattractant on ACE2 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Abnova

Product Name:

ACE2 polyclonal antibody

Catalog Number:

PAB13444

Clone Number:

N/A

This protein is encoded by angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerablehomology to human angiotensin 1 converting enzyme.This secreted protein catalyzes the cleavage ofangiotensin I into angiotensin and angiotensin II into the vasodilator angiotensin . It also play a major ole in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. Here we checked the time course effect of chemoattractant on ACE2 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We observed increased ACE2 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great for western blot. Signal is very strong. Highly recommended.

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