Anti-human CXCR5 from BD Biosciences Works Ok

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BV510 Rat Anti-Human CXCR5 (CD185)

Catalog Number:

563105

Clone Number:

RF8B2

Info:Supplier Page

Browse Similar Products: CXCR5 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CXCR5 in isolated PBMCs of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

anti-CXCR5 BV510 (1/10), 4°C, 20'

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We could stain CXCR5, although the staining is not very bright.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works.

The Bad

Not very bright

The Bottom Line

Can be used.

Share your experience with other scientists. Write a Review! »

Join the discussion