Reliable Antibody for Staining for Human CD172a from Biolegend

August 03, 2020

Pediatrics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-human CD172a

Catalog Number:

372106

Clone Number:

15-414

This product was used to compare the expression of CD172a on human neutrophils from blood and airways.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood and BAL fluid

Primary Incubation

2.5 uL of antibody added to 50 uL of blood or 2x10^5 BALF cells in 50 uL

Blocking Agent

Fc receptor blocking prior to primary incubation

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

R 670/30 filter on BD FACSYMPHONY

Results Summary

Blood and BAL were obtained from a CF patient for analysis of CD172a expression in blood and airway neutrophil populations. In blood neutrophils have uniform expression of this receptor. In the airways several populations are observed with differing levels of CD172a expression.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It's able to distinguish between populations with differing expression levels.

The Bad

Nothing.

The Bottom Line

Great antibody for studying phagocytic pathways.

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