BIO-RAD
iScript select cDNA synthesis kit
170-8897
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Quantitative gene expression using real-time PCR requires cDNA from the experimental sample. The RNA needs to be converted into cDNA. BIORAD script select provides us with an efficient reverse transcriptase to convert mRNA to cDNA which can be used for downstream processes
Quantitative real time PCR for gene expression studies
RNA
Do not exceed 1 microgram of RNA as the starting material
The Reverse transcriptase is very efficient with one microgram of the RNA good quality of cDNA is obtained. a 20 microliter reaction is more than enough to analyze more than 20 genes .
N/A
Separate oligo dt and random hexamer are provided in the kit
No drawback
I recommend this kit for quantitative gene expression studies across the test samples