Super Sensitive GAPDH Antibody from Sigma

March 26, 2020

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

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Quality of Results

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Company:

MilliporeSigma

Product Name:

GAPDH Antibody

Catalog Number:

ABS16

Clone Number:

Polyclonal

Browse Similar Products: GAPDH Western Blot Antibodies

I used this antibody to detect the expression of GAPDH in mouse dorsal root ganglion by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:100000 overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:10000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The recommended concentration for this antibody is 1:100000, so we always prepare a 1:10000 working solution for routine use. This antibody is really a saving of money and excellent in quality.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Super sensitive

The Bad

N/A

The Bottom Line

I strongly recommend this GAPDH antibody.

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