Good Fibroblast Marker from Sigma

January 10, 2020

Pharmaceutical and Biomedical Sciences
College of Pharmacy
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Monoclonal Anti-COL1A1 antibody produced in mouse

Catalog Number:

SAB1402151

Clone Number:

3G3

We work to evaluate the role of CNS basement membrane proteins in blood–brain barrier physiology and stroke pathology. We use this antibody to stain fibroblast cell population in the brain.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:200

Blocking Agent

Blocking Buffer (BSA, Donkey Serum and Triton X100)

Secondary Incubation

1:400

Tertiary Incubation

2 hr

Detection

Immunofluorescence

Results Summary

Excellent staining of meningeal fibroblast.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works really well

The Bad

Nothing

The Bottom Line

Recommended

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