Very Good Monomethyl-Histone H3 Antibody from Sigma

September 04, 2019

BlueskyReddit
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-monomethyl-Histone H3 (Lys27) Antibody

Catalog Number:

07-448

Clone Number:

Polyclonal

My research focuses on understanding how neurons generate their complex morphology and form proper circuitries during development, and how neurons regenerate to restore connections after brain or spinal cord injuries. I want to detect the expression of H3K27Me in mouse CAD cell line.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CAD cells

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The band is specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is ok

The Bad

N/A

The Bottom Line

It is ok for western detection of the expression of H3K27ME.

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