Klf4 Antibody from Sigma Is Very Good

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-Krueppel-Like Factor 4 (Klf4) Antibody

Catalog Number:

ABS1514

Clone Number:

1E6

Browse Similar Products: Klf4 Immunofluorescence Antibodies

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of Klf4 in mouse dorsal root ganglion.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:500, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The band looks clean and clear. However, as the instruction manual shows that the band is around 55 kDa, I observed that its molecular weight is quite near 62 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody is specific and strong.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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