MilliporeSigma
Anti-trimethyl Histone H3 (Lys27) Antibody, clone 18E9.1
05-1951
18E9.1
Browse Similar Products: Trimethyl Histone H3 Western Blot Antibodies
My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of H3K27me3 in mouse dorsal root ganglion.
Western Blot
Mouse dorsal root ganglion
1:5000, overnight at 4°C
TBST (0.1%) containing 5% blotting-grade blocker
1:2000, 1 hr at room temperature
N/A
KwikQuantTM Imager
There are two bands but only the lower one is H3K27me3 (MW 17).
The antibody is ok
There is an unspecific band
It is ok for detecting the expression of H3K27me3.